Project information
Plant sources of benzophenanthridine alkaloids and effects of these alkaloid on normal and cancer cells
- Project Identification
- GA525/04/0017
- Project Period
- 1/2004 - 1/2006
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
-
Faculty of Science
- prof. RNDr. Eva Táborská, CSc.
- RNDr. Hana Bochořáková
- doc. RNDr. Jiří Dostál, CSc.
- prof. RNDr. Radek Marek, Ph.D.
- Ing. Pavel Musil
- RNDr. Hana Paulová, CSc.
- prof. MUDr. Iva Slaninová, Ph.D.
- Keywords
- benzophenanthridine, Papaveraceae, antitumor, cytotoxicity, cytoskeleton, apoptosis
Our rent paper (Slaninová et.al., 2001) reported the effects of four quaternary benzophenanthridine alakloids (QBA) on human tumor (HeLa) cells an two yeast species (Saccaromyces serevisiace, Schizosaccharomyces japonicus). We had only limite amounts of QBA from the collection of lakaloids at Department of Biochemistry LF MU Brno. The aim of this project is to cultivate selected plant species from the tribe Chelidonieae (Papaveraceae) which contain minor QBA. The cultivation wil be perormed in Centre of Medicinal Plants Medical Faculty MU. The following task is to determine the best plant source of these minor QBA and to develop optimal cultivation method. Our next aim is to identify new compounds which display biological activities, expecially on cell proliferation, to compare their effects on human (normal and cancer) and yeast cells, to attempt to explain a mechanism of their activities. Tests assessing the cytotoxicity, antiproliferative activity and apoptosis will be used. The effects on the cell morphology, cytoskeleton and the dynamics of alkaloids in cells will be used. The effects on the cell morphology, cytoskeleton and the dynamics of alkaloids in cells will be studied using fluorescent microscopy (indirect immunofluorescence). The changes on the ultrastructural level will be followed using electron microschopy. On molecular level, in vitro interactions of some quaternary alkaloids with bases of nucleic acids, nucleotides and oligonucleotides will be studied by NMR spectroschopy and X-ray diffraction analysis.
Publications
Total number of publications: 32
2006
-
Benzophenanthridine alkaloids as a new supravital DNA dyes.
Sborník XIV. Cytoskeletálního klubu, year: 2006
-
Evaluation of benzophenanthridine alkaloids content in the selected species of family Papaveraceae
Chemistry, Pharmacology and Biosynthesis of Alkaloids, year: 2006
-
New findings of minority benzo[c]phenenthridine alkaloids
Book of abstracts of CHEMISTRY, PHARMACOLOGY AND BIOSYNTHESIS OF ALKALOIDS, year: 2006
-
NMR study of protoberberines and benzophenanthridines
XXXIX OGÓLNOPOLSKIEGO SEMINARIUM NA TEMAT MAGNETYCZNEGO REZONANSU JADROWEGO I JEGO ZASTOSOWAN, year: 2006
-
Nukleofilní adice na protoberberinový a benzofenanthridinový skelet
Chemické listy, year: 2006
-
Plant sources of minor benzo[c]phenanthridine alkaloids
Future trends in Phytochemistry, year: 2006
-
Použití makarpinu ke klasifikaci buněk průtokovou cytometrií.
Publisher: Úřad průmyslového vlastnictví, state: Czech Republic, patent's number: 297482, year: 2006
-
Studium biologických efektů benzo[c]phenanthridinových alkaloidů na nádorových a nenádorových buněčných liniích
Sborník 50.studentské vědecké konference LF MU, year: 2006
-
The cytotoxicity of minority benzo[c]phenanthridine alkaloids
Books of abstracts of CHEMISTRY, PHARMACOLOGY AND BIOSYNTHESIS OF ALKALOIDS, year: 2006
2005
-
A Quantum Chemical Study vs. Biological Activity of Seven Quaternary Benzophenanthridine Alkaloids
11th Blue Danube Symposium on Heterocyclic Chemistry - Book of Abstracts, year: 2005