Project information
Single cell analysis: a modern tool to study clonal evolution in high-risk patients with chronic lymphocytic leukemia (Single cell analysis of CLL cells)

Information

This project doesn't include Faculty of Science. It includes Central European Institute of Technology. Official project website can be found on muni.cz.

Project Identification

NU20-08-00314

Project Period

5/2020 - 12/2023

Investor / Pogramme / Project type

Ministry of Health of the CR

Ministry of Health Research Programme 2020 - 2026
Subprogram 1 - standard

MU Faculty or unit

Central European Institute of Technology

prof. RNDr. Šárka Pospíšilová, Ph.D.

Cooperating Organization

University Hospital Brno-Bohunice

Responsible person Mgr. Jana Kotašková, Ph.D.

The proposed project builds on continuous well established research of our team in the field of hematologic malignancies. In this study we would like to profound our knowledge and understanding the mechanisms underlying clonal evolution of chronic lymphocytic leukaemia (CLL) cells. We have already studied CLL using variety of bulk analysis moreover we have also succeeded in single cell analysis. We believe that true personalised medicine is based of detailed characterisation of malignant cells of each patient with the potential to detect the most aggressive clones. From this prospective, single cell analysis gives the best opportunity to fulfil this need. The aim of our study is to compare transcriptomes of thousands CLL cells from consecutively collected samples of patients with aggressive disease with the aim to define the properties of subclones responsible for disease refractoriness.

Sustainable Development Goals

Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.

Publications

Total number of publications: 20

2024

Integrative NGS testing reveals clonal dynamics of adverse genomic defects contributing to a natural progression in treatment-naïve CLL patients

NAVRKALOVÁ Veronika PLEVOVÁ Karla RADOVÁ Lenka PORC Jakub Paweł PÁL Karol MALČÍKOVÁ Jitka PAVLOVÁ Šárka DOUBEK Michael PANOVSKÁ Anna KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka

Article in Periodical

British journal of haematology, year: 2024, volume: 204, edition: 1, DOI
IκBε deficiency accelerates disease development in chronic lymphocytic leukemia

BORDINI Jessica LENZI Chiara FRENQUELLI Michela MORABITO Alessia PSEFTOGAS Athanasios BELLONI Daniela MANSOURI Larry TSIOLAS George PEROTTA Eleonora RANGHETTI Pamela GANDINI Francesca GENOVA Francesca HAGERSTRAND Daniel GAVRIILIDIS Georgios KEISARIS Sofoklis PECHLIVANIS Nikolaos DAVI Frederic KAY Neil E LANGERAK Anton W POSPÍŠILOVÁ Šárka SCARFO Lydia MAKRIS Antonios PSOMOPOULOS Fotis E STAMATOPOULOS Kostas ROSENQUIST Richard CAMPANELLA Alessandro GHIA Paolo

Article in Periodical

Leukemia, year: 2024, volume: 38, edition: 6, DOI
Unveiling the dynamics and molecular landscape of a rare chronic lymphocytic leukemia subpopulation driving refractoriness: insights from single-cell RNA sequencing

KURUCOVÁ Terézia RÉBLOVÁ Kamila JANOVSKÁ Pavlína PORC Jakub Paweł NAVRKALOVÁ Veronika PAVLOVÁ Šárka MALČÍKOVÁ Jitka PLEVOVÁ Karla TICHÝ Boris DOUBEK Michael BRYJA Vítězslav KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka

Article in Periodical

Molecular oncology, year: 2024, volume: 18, edition: 10, DOI

2023

A web-based bioinformatic tool LYNX for targeted LYmphoid NeXt- generation sequencing data analysis and visualization for hematooncology.

REIGL Tomáš PORC Jakub Paweł NAVRKALOVÁ Veronika HYNŠT Jakub PÁL Karol STRÁNSKÁ Kamila KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka PLEVOVÁ Karla

Conference abstract

Year: 2023, type: Conference abstract
Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development

VÁŇOVÁ Tereza SEDMÍK Jiří RAŠKA Jan AMRUZ ČERNÁ Kateřina TAUŠ Petr POSPÍŠILOVÁ Veronika NEZVEDOVÁ Markéta FEDOROVÁ Veronika KADÁKOVÁ Soňa KLÍMOVÁ Hana CAPANDOVÁ Michaela ORVISKÁ Petra FOJTÍK Petr BÁRTOVÁ Simona PLEVOVÁ Karla SPÁČIL Zdeněk HŘÍBKOVÁ Hana BOHAČIAKOVÁ Dáša

Article in Periodical

CELL REPORTS, year: 2023, volume: 42, edition: 11, DOI
RNA sekvenovanie jednotlivých buniek umožňuje charakterizáciu a sledovanie zriedkavých CLL buniek s potenciálom navodiť refraktérne ochorenie.

KURUCOVÁ Terézia RÉBLOVÁ Kamila VALIŠOVÁ Martina PORC Jakub Paweł NAVRKALOVÁ Veronika ZÁVACKÁ Kristýna PLEVOVÁ Karla TICHÝ Boris DOUBEK Michael KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2023, type: Conference abstract
Single-cell RNA-sequencing enables tracking and characterization of rare CLL cells with the potential to cause refractoriness.

KURUCOVÁ Terézia RÉBLOVÁ Kamila VALIŠOVÁ Martina PORC Jakub Paweł NAVRKALOVÁ Veronika PAVLOVÁ Šárka MALČÍKOVÁ Jitka PLEVOVÁ Karla TICHÝ Boris DOUBEK Michael KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2023, type: Conference abstract
Subclonal architecture of chromosomes revealed by single-cell analysis of gene expression in a patient with clonal evolution of relapsing/refractory CLL.

KOTAŠKOVÁ Jana KURUCOVÁ Terézia RÉBLOVÁ Kamila ZÁVACKÁ Kristýna MAREČKOVÁ Andrea BOHÚNOVÁ Michaela NAVRKALOVÁ Veronika JAROŠOVÁ Marie DOUBEK Michael PLEVOVÁ Karla POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2023, type: Conference abstract
Tracking CLL cells with aberrations in the TP53 gene using scRNA-seq in relapsed/refractory patients.

NAVRKALOVÁ Veronika KURUCOVÁ Terézia RÉBLOVÁ Kamila BOHÚNOVÁ Michaela PLEVOVÁ Karla DOUBEK Michael MALČÍKOVÁ Jitka PAVLOVÁ Šárka KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2023, type: Conference abstract

2022

Cytogenetics in Chronic Lymphocytic Leukemia: ERIC Perspectives and Recommendations

BALIAKAS Panagiotis ESPINET Blanca MELLINK Clemens JAROŠOVÁ Marie ATHANASIADOU Anastasia GHIA Paolo KATER Arnon P OSCIER David HAFERLACH Claudia STAMATOPOULOS Kostas

Article in Periodical

HemaSphere, year: 2022, volume: 6, edition: 4, DOI