Project information
In vitro methods for detection and studies of epigenetic toxicity of xenobiotics
- Project Identification
- GA525/00/D101
- Project Period
- 1/2000 - 1/2003
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
Polycyclic aromatic hydrocarbons (PAHs), pesticides and other compounds belong among the most important environmental pollutants. Although many mechanisms of their toxic effects were recognized (mutagenicity, immunotoxicity), new evidences show that mech anisms of epigenetic toxicity affecting processes of cancerogenesis can be involved. Project propose development, evaluation and application of the battery of in vitro tests for detection of epigenetic mechanisms of cellular and molecular toxicity. Some of these tests are still omitted of completely missing. Following are the in vitro tests included in the proposed battery: multiparametric system for studies of oxidative stress (ROS production, GSH depletion, oxidative DNA damage, lipid peroxidation), a pplication of the methods of receptor-mediated toxicity (AhR, ER) and development of new methods (PPAR), research of inhibitors of intercellular gap juntional communications (GJIC) and development of models for studies of detoxifying enzymes (CYPIA, CYP1
Publications
Total number of publications: 23
2002
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In vitro biotesty pro detekci negenotoxických procesů - studie efektů PAHs a hydroxyderivátů PCBs
Sborník pracovní konference - Ekotoxikologické Biotesty I, Seč u Chrudimi, 18-19/9/2002, year: 2002
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Inhibition of gap junctional intercellular communication and the activation of mitogen activated protein kinase by PCBs depends on substitution of chlorine ortho-position
Book of Abstracts - The 2nd PCB Workshop: Recent Advances in the Environmental Toxicology and Health Effects of PCBs Emphasis on the latest advances and the perspective of Central and Eastern Europe. Brno, CR, 07-11/05/2002, year: 2002
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Inhibition of gap junctional intercellular communication and the activation of mitogen activated protein kinase by PCBs depends on substitution of chlorine ortho-position
The Second PCB Workshop. Recent Advances in the Environmental Toxicology and Health Effects of PCBs. Book of Abstracts, year: 2002
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Inhibition of GJIC by polychlorinated biphenyls: inhibitory potencies and screening for potential mode(s) of action
SECOTOX 2002, Trends and Advances in Environmental Chemistry and Ecotoxicology, Book of Abstracts, year: 2002
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Non-genotoxic effects of hydroxy-PCBs and PCB-quinones in liver and mammary in vitro models
Book of Abstracts - The 2nd PCB Workshop: Recent Advances in the Environmental Toxicology and Health Effects of PCBs Emphasis on the latest advances and the perspective of Central and Eastern Europe. Brno, CR, 07-11/05/2002, year: 2002
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Nongenotoxic modes of action of polycyclic aromatic hydrocarbons and their relative potencies in rat liver cellular models. Ecotoxicological significance of individual compounds in abiotic samples
Book of Abstracts - SECOTOX 2002, Trends and Advances in Environmental Chemistry and Ecotoxicology, Brno, 14-16/10/2002, year: 2002
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Relative potencies of oxygenated polychlorinated biphenyls to inhibit gap-junctional intercellular communication in WB-F344 cells
Book of Abstracts - The 2nd PCB Workshop: Recent Advances in the Environmental Toxicology and Health Effects of PCBs Emphasis on the latest advances and the perspective of Central and Eastern Europe. Brno, CR, 07-11/05/2002, year: 2002
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Screening of the health risks of biofilms using a battery of in vitro toxicity assays
Book of Abstracts - SECOTOX 2002, Trends and Advances in Environmental Chemistry and Ecotoxicology, Brno, 14-16/10/2002, year: 2002
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Vliv nově syntetizovaných chalkonů na epigenetické faktory karcinogeneze
Chemické listy, year: 2002, volume: 96, edition: 6
2001
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Aryl hydrocarbon receptor-mediated and estrogenic activities of oxygenated polycyclic aromatic hydrocarbons and azaarenes originally identified in extracts of river sediments
Environmental Toxicology and Chemistry, year: 2001, volume: 20, edition: 12