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Project information
Transkription activation system for studying the relationship between cytokinin metabolism and action in Arabidopsis and tobacco

Project Identification
IAA5004001
Project Period
1/2000 - 1/2002
Investor / Pogramme / Project type
Academy of Sciences of the Czech Republic
  • Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
MU Faculty or unit
Faculty of Science
  • RNDr. Alena Kuderová, CSc.
Cooperating Organization
Institute of Biophysics
  • Responsible person prof. RNDr. Břetislav Brzobohatý, CSc.
Institute of Experimental Botany of the ASCR, v. v. i.
  • Responsible person RNDr. Ivana Macháčková, CSc.

Cytokinins (CKs) are known to act in combination with other phytohormones to regulate diverse developmental processes in plants. However, knowledge of molecualr mechanism of CK action is limited. We will use a novel transcription activation system for regulated gene expression to create transgenic plants overproducing individually heterologous enzymes generating free CKs either by glucoside hydrolysis or by de novo synthesis in defined spatial and temporal patterns. We expect that CK release from glucosides will cause. in addition to common effects. several distrinct effects compared to de novo CK synthesis. Analysis of the distinct effects will contribute to understanding of complexity of CK metobolic conversions. This approach will lead to isolation of Arabidopsis lines displaying phenotypes corresponding to defined CK overproduction patterns. In future, the lines will be used, via isolation of suppresor mutations, to isolate genes involved in CK metabolism, and CK signal pathways.

Publications

Total number of publications: 19

2002

2001

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