Project information
Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
- Project Identification
- GV204/97/K084
- Project Period
- 1/1997 - 1/2001
- Investor / Pogramme / Project type
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Czech Science Foundation
- Complex Projects
- MU Faculty or unit
- Faculty of Science
- Cooperating Organization
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Institute of Biophysics
- Responsible person prof. RNDr. Emil Paleček, DrSc.
- Responsible person doc. Ing. Ladislav Novotný, CSc.
- Responsible person RNDr. Bořivoj Vojtěšek, DrSc.
This project of complex studies of biomacromolecule interactions with electrically charged surfaces is based on the recent progress reached in the proposers' laboratories (see the text) in the development of the electrochemical techniques and in the stud ies of interactions of nucleic acids and proteins with the mercury electrode and solid surfaces. In this project basic information about the adsorption/desorption, redox properties and structural features of DNA, RNA, oligonucleotides, proteins and small peptides at interfaces will be obtained. As a result of these studies electrochemical ultramicromethods will be developed including (a) fast response sensors of the DNA damage (b) fast response sensors of the DNA hybridization c new methods for studies of the interaction of DNA with specific proteins and anticancer drugs, etc. In addition information about the DNA local structures in neurological genetic diseases and about the DNA-p53 protein interactions in human cancer will be obtained. The main goal
Publications
Total number of publications: 83
2002
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Stanovení azidothymidinu v lidské moči, krevním séru a krvi
Sborník z VI. pracovního setkání biochemiků a molekulárních biologů, year: 2002
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Stanovení glutathion-S-transferasy na pevných elektrodách
Sborník abstrakt III. pracovního setkání fyzikálních chemiků a elektrochemiků v Brně, year: 2002
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Thin film mercury electrodes in electrochemical analysis of the nucleic acids and their componends
Book of Abstracts, p.83, year: 2002
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Two-dimensional condensation of nucleic acid components at mercury film and gold electrodes.
Bioelectrochemistry, year: 2002, volume: 56, edition: 1-2
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Use of artificial neural networks for the evaluation UV-Vis spectra of adenine and cytosine in mixtures
Chemické listy, year: 2002
2001
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Adsorption of polyvinylsulfate and polysodium 4-styrene sulfonate at the mercury electrode.
Book of Abstracts, XVI th International Symposium on Bioelectrochemistry and Bioenergetics, year: 2001
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Bakterie Escherichia Coli a střídavá nízkofrekvenční magnetická pole; závislost na frekvenci, pokus o synchronizaci buněčné kultury, kombinace účinků magnetického pole a UV záření.
Book of Abstracts. XXIV.dny lékařské biofyziky, year: 2001
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Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry
Anal. Chem., year: 2001, volume: 2001, edition: 20
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Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).
Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001, year: 2001
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Electrochemical impedance spectroscopy of polynucleotides.
Book of Abstracts, XVI th International Symposium on Bioelectrochemistry and Bioenergetics, year: 2001