Project information
Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
- Project Identification
- GV204/97/K084
- Project Period
- 1/1997 - 1/2001
- Investor / Pogramme / Project type
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Czech Science Foundation
- Complex Projects
- MU Faculty or unit
- Faculty of Science
- Cooperating Organization
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Institute of Biophysics
- Responsible person prof. RNDr. Emil Paleček, DrSc.
- Responsible person doc. Ing. Ladislav Novotný, CSc.
- Responsible person RNDr. Bořivoj Vojtěšek, DrSc.
This project of complex studies of biomacromolecule interactions with electrically charged surfaces is based on the recent progress reached in the proposers' laboratories (see the text) in the development of the electrochemical techniques and in the stud ies of interactions of nucleic acids and proteins with the mercury electrode and solid surfaces. In this project basic information about the adsorption/desorption, redox properties and structural features of DNA, RNA, oligonucleotides, proteins and small peptides at interfaces will be obtained. As a result of these studies electrochemical ultramicromethods will be developed including (a) fast response sensors of the DNA damage (b) fast response sensors of the DNA hybridization c new methods for studies of the interaction of DNA with specific proteins and anticancer drugs, etc. In addition information about the DNA local structures in neurological genetic diseases and about the DNA-p53 protein interactions in human cancer will be obtained. The main goal
Publications
Total number of publications: 83
2001
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Stanovení metallothioneinu v tkáních okouna říčního a kapra obecného(Perca fluviatilis L., Cyprinus carpio L.)
Sborník:5.pracovní setkání biochemiků a molekulárních biologů, Brno 2001, year: 2001
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Stanovení MT a těžkých kovů spektrální a elektrochemickou metodou v různých tkáních
Moderní elektrochemické metody, year: 2001
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Stanovení těžkých kovů v tkáních Okouna říčního (Perca fluviatilis L.) a Kapra obecného (Cyprinus carpio L.)
V. Pracovní setkání biochemiků a molekulárních biologů, year: 2001
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Studium elektrodového procesu pomocí eliminačních metod
Moderní elektrochemické metody, year: 2001
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Studium interakcí nukleových kyselin s povrchy modifikovaných elektrod elektrochemickou impedanční spektroskopií.
Book of Abstracts. XXIV.dny lékařské biofyziky, year: 2001
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Two-dimensional condensation of nucleic acid components at mercury film and gold electrodes
Book of Abstracts, XVI th International Symposium on Bioelectrochemistry and Bioenergetics, year: 2001
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Use of artificial neural networks for the evaluation of electrochemical signals of adenine and cytosine in mixtures
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, year: 2001, volume: 2001, edition: 503
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Use of artificial neural networks for the evaluation of electrochemical signals of adenine and cytosine in mixtures interfered with hydrogen evolution
J.Electroanal.Chem., year: 2001, volume: 2002, edition: 1-2
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Vliv selenu, zinku a kadmia na růstový vývoj česneku kuchyňského
53.zjazd chemických spoločností, Banská Bystrica, Slovensko, year: 2001
2000
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ADSORPTIVE STRIPPING ANALYSIS OF DAMAGE DNA WITH ADMITTANCE MEASUREMENTS
Book of Abstracts, J.Heyrovský Memorial Symposium, Praha, year: 2000